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Effects of Feeding OdorLess

Effects of Feeding OdorLess™ ** to Eliminate Dog Feces Odor

By Chanin Tirawattanawanich1* and Siwa Peerakeatpajon1

In the experiments, dogs ate OdorlessTM compared to other food supplements. Those who did not eat food supplement tablets or OdorlessTM found within one week that the amount of chemicals that cause odor, such as Protein Metabolite, Ammonia Nitrogen and Sulfide, decreased significantly (p <. 001) and had no effect in reducing Indole significantly (p>0.05). The Carbohydrate metabolite decreased due to OdorlessTM and grain supplements (p <0.05) with no significant difference between the two groups (p>0.15).

Emission of odors from livestock is caused by gas generated and emitted by the animal, and the odor caused by the decomposition of waste excreted by the animals, especially that found in animal manure. The buildings housing the livestock smell badly, which is a problem for the owners, people who also live there and neighbors closeby.
In addition, these gases are pollutants, and also possibly harmful to human physical and mental health (Schiffman, 1998; Rideout et al, 2004; Swanson et al., 2002). They also have a negative effect on the digestive system and throughout the body of the animal itself. The main chemical compounds causing odors are ammonia, indoles, skatole, phenols (phenol, p-cresol) and volatile sulfur-containing compounds such as dimethyl disulfide, diethyl disulfide, di-n-propyl disulfide and di-n-butyl disulfide. These compounds are known to cause gastrointestinal health problems, especially the end colon (Hussein et al., 1999; Johnson, 1977; Silverman and Andrews, 1977). Ammonium compounds can also affect birth and cause tumors (Lin and Visek, 1991; Visek, 1978). Skatole and indole are associated with cancer and tumors in the gastrointestinal tract. Indole is used to measure cancer conditions (Karlin et al., 1984),
and findings also indicate Indole can cause tissue damage
from the digestive oxidation process of the gastrointestinal tract with the colon (Garbe et al., 1999).
A previous study using Fructo-oligosaccharide or FOS supplements in the diet showed a decrease in substances produced from the breakdown of proteins. Volatile sulfide compounds in the group also enhanced the digestive system within the gastrointestinal tract as well (Swanson et al., 2002).
Bacillus subtilis is a bacterium found naturally in the soil. Bacteria in the experiments were found to grow and produce spores. In experiments with chickens (Tam et al., 2006) the feeding spores of Bacillus subtilis were also able to occupy and grow within the anaerobic conditions found in the gastrointestinal tract and within the intestines and colons of chickens, (Stephen et al., 2008)indicating the possibility that Bacillus subtilis can work within the digestive system of dogs with lower oxygen content.
According to experiments by Yumoto et al, a reduction in the smell of manure was achieved by using Bacillus asahii to reduce the amount of fatty acid vapors causing the smell. (Yumoto et al., 2004).
1Department of Physiology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand
* Corresponding author, e-mail: fvetcnt@ku.ac.th
**Odorless™ Probiotic and Prebiotic products group of White Crane (V 88) Aqua-Tech Ltd., in experiments certifying the reduction in smells from body odor, saliva and feces produced by digestive system/ gastrointestinal function using Fructo-oligosaccharide (FOS) and Bacillus spp.
These trials tested the effects of animals eating OdorlessTM (which is a mixture of Bacills spp) to reduce the toxic odors in dog feces compared with eating normal food and food supplements that do not have Bacillus spp present.
In these experiments, OdorlessTM effectively reduced the smell of dog feces by reducing the odor caused by toxins such as Ammonia nitrogen, Sulfide and Carbohydrate metalite but not the amount of Indole. Changes effected by the Bacillus spp will not necessarily reduce the amount of Indole. However, these changes are more likely to be caused by other substances used in food supplements or as a result of the FOS itself, as the trials did not detect an increase in Skatole or Indole. This may be due to the volume of Skatole present being lower after extraction than the test equipment was able to detect (at least ppm), which could be a topic for further study.


Documents and references
Garbe T.R., M. Kobayashi, H. Yukawa. 2000. Indole-inducible proteins in bacteria suggest membrane and oxidant toxicity. Archieves Microbiology. 173:78–82.
Hussein H.S., E.A. Flickinger and G.C. Fahey Jr. 1999. Petfood Applications of Inulin and Oligofructose. J. Nutrition. 129: 1454S–1456S. Fructooligosaccharides and Lactobacillus acidophilus Modify Gut Microbial Populations, Total Tract Nutrient Digestibilities and Fecal Protein Catabolite Concentrations in Healthy Adult Dogs. J. Nutrition. 132: 3721–3731.
Karlin D.A., A.J. Mastromarino, R.D. Jones, J.R. Stroehlein and O. Lorentz. 1984. Fecal skatole and indole and breath methane and hydrogen in patients with large bowel polyps or cancer. J Cancer Res. Clinic Oncology. 109:135-141.
Lin H.C. and W.J. Visek. 1991. Large intestinal pH and ammonia in rats: dietary fat and protein interactions. J. Nutrition. 121: 832–843.
Schiffman S.S. 1998. Livestock Odors: Implications for Human Health and Well-Being. J. Animal. Sci. 76:1343–1355.
Silverman S.J. and A.W. Andrews. 1977. Bile acids, comutagenic activity in the Salmonella-mammalian microsome mutagenicity test. J. Nat. Cancer Inst. 59:1557–1559.
Stephen T.C., M.R. Roberto and J.W. Martin. 2008. Bacillus subtilis Spores Germinate in the Chicken Gastrointestinal Tract. Applied and Environmental Microbiology. 74(16): 5254-5258.
Swanson K.S., C.M. Grieshop, E.A. Flickinger, L.L. Bauer, J. Chow, B.W. Wolf, K.A. Garleb and G.C. Fahey, Jr. 2002.
Tam N.K., N.Q. Uyen, H.A. Hong, Le H. Duc, T.T. Hoa, C.R. Serra, A.O. Henriques, S.M. Cutting. 2006. The Intestinal Life Cycle of Bacillus subtilis and Close Relatives. J. Bacteriology. 188(7): 2692–2700.
Yumoto I., K. Hirota , Y. Nodasaka, Y. Tokiwa and K. Nakajima. 2004. Bacillus asahii sp. nov., a novel bacterium isolated from soil with the ability to deodorize the bad smell generated from short-chain fatty acids. International journal of systematic and evolutionary microbiology. 54(6):1997-2001.Effects of Feeding OdorLess™ ** to Eliminate Dog Feces Odor

By Chanin Tirawattanawanich1* and Siwa Peerakeatpajon1

In the experiments, dogs ate OdorlessTM compared to other food supplements. Those who did not eat food supplement tablets or OdorlessTM found within one week that the amount of chemicals that cause odor, such as Protein Metabolite, Ammonia Nitrogen and Sulfide, decreased significantly (p <. 001) and had no effect in reducing Indole significantly (p>0.05). The Carbohydrate metabolite decreased due to OdorlessTM and grain supplements (p <0.05) with no significant difference between the two groups (p>0.15).

Emission of odors from livestock is caused by gas generated and emitted by the animal, and the odor caused by the decomposition of waste excreted by the animals, especially that found in animal manure. The buildings housing the livestock smell badly, which is a problem for the owners, people who also live there and neighbors closeby.
In addition, these gases are pollutants, and also possibly harmful to human physical and mental health (Schiffman, 1998; Rideout et al, 2004; Swanson et al., 2002). They also have a negative effect on the digestive system and throughout the body of the animal itself. The main chemical compounds causing odors are ammonia, indoles, skatole, phenols (phenol, p-cresol) and volatile sulfur-containing compounds such as dimethyl disulfide, diethyl disulfide, di-n-propyl disulfide and di-n-butyl disulfide. These compounds are known to cause gastrointestinal health problems, especially the end colon (Hussein et al., 1999; Johnson, 1977; Silverman and Andrews, 1977). Ammonium compounds can also affect birth and cause tumors (Lin and Visek, 1991; Visek, 1978). Skatole and indole are associated with cancer and tumors in the gastrointestinal tract. Indole is used to measure cancer conditions (Karlin et al., 1984),
and findings also indicate Indole can cause tissue damage
from the digestive oxidation process of the gastrointestinal tract with the colon (Garbe et al., 1999).
A previous study using Fructo-oligosaccharide or FOS supplements in the diet showed a decrease in substances produced from the breakdown of proteins. Volatile sulfide compounds in the group also enhanced the digestive system within the gastrointestinal tract as well (Swanson et al., 2002).
Bacillus subtilis is a bacterium found naturally in the soil. Bacteria in the experiments were found to grow and produce spores. In experiments with chickens (Tam et al., 2006) the feeding spores of Bacillus subtilis were also able to occupy and grow within the anaerobic conditions found in the gastrointestinal tract and within the intestines and colons of chickens, (Stephen et al., 2008)indicating the possibility that Bacillus subtilis can work within the digestive system of dogs with lower oxygen content.
According to experiments by Yumoto et al, a reduction in the smell of manure was achieved by using Bacillus asahii to reduce the amount of fatty acid vapors causing the smell. (Yumoto et al., 2004).
1Department of Physiology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand
* Corresponding author, e-mail: fvetcnt@ku.ac.th
**Odorless™ Probiotic and Prebiotic products group of White Crane (V 88) Aqua-Tech Ltd., in experiments certifying the reduction in smells from body odor, saliva and feces produced by digestive system/ gastrointestinal function using Fructo-oligosaccharide (FOS) and Bacillus spp.
These trials tested the effects of animals eating OdorlessTM (which is a mixture of Bacills spp) to reduce the toxic odors in dog feces compared with eating normal food and food supplements that do not have Bacillus spp present.
In these experiments, OdorlessTM effectively reduced the smell of dog feces by reducing the odor caused by toxins such as Ammonia nitrogen, Sulfide and Carbohydrate metalite but not the amount of Indole. Changes effected by the Bacillus spp will not necessarily reduce the amount of Indole. However, these changes are more likely to be caused by other substances used in food supplements or as a result of the FOS itself, as the trials did not detect an increase in Skatole or Indole. This may be due to the volume of Skatole present being lower after extraction than the test equipment was able to detect (at least ppm), which could be a topic for further study.


Documents and references
Garbe T.R., M. Kobayashi, H. Yukawa. 2000. Indole-inducible proteins in bacteria suggest membrane and oxidant toxicity. Archieves Microbiology. 173:78–82.
Hussein H.S., E.A. Flickinger and G.C. Fahey Jr. 1999. Petfood Applications of Inulin and Oligofructose. J. Nutrition. 129: 1454S–1456S. Fructooligosaccharides and Lactobacillus acidophilus Modify Gut Microbial Populations, Total Tract Nutrient Digestibilities and Fecal Protein Catabolite Concentrations in Healthy Adult Dogs. J. Nutrition. 132: 3721–3731.
Karlin D.A., A.J. Mastromarino, R.D. Jones, J.R. Stroehlein and O. Lorentz. 1984. Fecal skatole and indole and breath methane and hydrogen in patients with large bowel polyps or cancer. J Cancer Res. Clinic Oncology. 109:135-141.
Lin H.C. and W.J. Visek. 1991. Large intestinal pH and ammonia in rats: dietary fat and protein interactions. J. Nutrition. 121: 832–843.
Schiffman S.S. 1998. Livestock Odors: Implications for Human Health and Well-Being. J. Animal. Sci. 76:1343–1355.
Silverman S.J. and A.W. Andrews. 1977. Bile acids, comutagenic activity in the Salmonella-mammalian microsome mutagenicity test. J. Nat. Cancer Inst. 59:1557–1559.
Stephen T.C., M.R. Roberto and J.W. Martin. 2008. Bacillus subtilis Spores Germinate in the Chicken Gastrointestinal Tract. Applied and Environmental Microbiology. 74(16): 5254-5258.
Swanson K.S., C.M. Grieshop, E.A. Flickinger, L.L. Bauer, J. Chow, B.W. Wolf, K.A. Garleb and G.C. Fahey, Jr. 2002.
Tam N.K., N.Q. Uyen, H.A. Hong, Le H. Duc, T.T. Hoa, C.R. Serra, A.O. Henriques, S.M. Cutting. 2006. The Intestinal Life Cycle of Bacillus subtilis and Close Relatives. J. Bacteriology. 188(7): 2692–2700.
Yumoto I., K. Hirota , Y. Nodasaka, Y. Tokiwa and K. Nakajima. 2004. Bacillus asahii sp. nov., a novel bacterium isolated from soil with the ability to deodorize the bad smell generated from short-chain fatty acids. International journal of systematic and evolutionary microbiology. 54(6):1997-2001.Effects of Feeding OdorLess™ ** to Eliminate Dog Feces Odor
By Chanin Tirawattanawanich1* and Siwa Peerakeatpajon1

In the experiments, dogs ate OdorlessTM compared to other food supplements. Those who did not eat food supplement tablets or OdorlessTM found within one week that the amount of chemicals that cause odor, such as Protein Metabolite, Ammonia Nitrogen and Sulfide, decreased significantly (p <. 001) and had no effect in reducing Indole significantly (p>0.05). The Carbohydrate metabolite decreased due to OdorlessTM and grain supplements (p <0.05) with no significant difference between the two groups (p>0.15).

Emission of odors from livestock is caused by gas generated and emitted by the animal, and the odor caused by the decomposition of waste excreted by the animals, especially that found in animal manure. The buildings housing the livestock smell badly, which is a problem for the owners, people who also live there and neighbors closeby.
In addition, these gases are pollutants, and also possibly harmful to human physical and mental health (Schiffman, 1998; Rideout et al, 2004; Swanson et al., 2002). They also have a negative effect on the digestive system and throughout the body of the animal itself. The main chemical compounds causing odors are ammonia, indoles, skatole, phenols (phenol, p-cresol) and volatile sulfur-containing compounds such as dimethyl disulfide, diethyl disulfide, di-n-propyl disulfide and di-n-butyl disulfide. These compounds are known to cause gastrointestinal health problems, especially the end colon (Hussein et al., 1999; Johnson, 1977; Silverman and Andrews, 1977). Ammonium compounds can also affect birth and cause tumors (Lin and Visek, 1991; Visek, 1978). Skatole and indole are associated with cancer and tumors in the gastrointestinal tract. Indole is used to measure cancer conditions (Karlin et al., 1984),
and findings also indicate Indole can cause tissue damage
from the digestive oxidation process of the gastrointestinal tract with the colon (Garbe et al., 1999).
A previous study using Fructo-oligosaccharide or FOS supplements in the diet showed a decrease in substances produced from the breakdown of proteins. Volatile sulfide compounds in the group also enhanced the digestive system within the gastrointestinal tract as well (Swanson et al., 2002).
Bacillus subtilis is a bacterium found naturally in the soil. Bacteria in the experiments were found to grow and produce spores. In experiments with chickens (Tam et al., 2006) the feeding spores of Bacillus subtilis were also able to occupy and grow within the anaerobic conditions found in the gastrointestinal tract and within the intestines and colons of chickens, (Stephen et al., 2008)indicating the possibility that Bacillus subtilis can work within the digestive system of dogs with lower oxygen content.
According to experiments by Yumoto et al, a reduction in the smell of manure was achieved by using Bacillus asahii to reduce the amount of fatty acid vapors causing the smell. (Yumoto et al., 2004).
1Department of Physiology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand
* Corresponding author, e-mail: fvetcnt@ku.ac.th
**Odorless™ Probiotic and Prebiotic products group of White Crane (V 88) Aqua-Tech Ltd., in experiments certifying the reduction in smells from body odor, saliva and feces produced by digestive system/ gastrointestinal function using Fructo-oligosaccharide (FOS) and Bacillus spp.
These trials tested the effects of animals eating OdorlessTM (which is a mixture of Bacills spp) to reduce the toxic odors in dog feces compared with eating normal food and food supplements that do not have Bacillus spp present.
In these experiments, OdorlessTM effectively reduced the smell of dog feces by reducing the odor caused by toxins such as Ammonia nitrogen, Sulfide and Carbohydrate metalite but not the amount of Indole. Changes effected by the Bacillus spp will not necessarily reduce the amount of Indole. However, these changes are more likely to be caused by other substances used in food supplements or as a result of the FOS itself, as the trials did not detect an increase in Skatole or Indole. This may be due to the volume of Skatole present being lower after extraction than the test equipment was able to detect (at least ppm), which could be a topic for further study.


Documents and references
Garbe T.R., M. Kobayashi, H. Yukawa. 2000. Indole-inducible proteins in bacteria suggest membrane and oxidant toxicity. Archieves Microbiology. 173:78–82.
Hussein H.S., E.A. Flickinger and G.C. Fahey Jr. 1999. Petfood Applications of Inulin and Oligofructose. J. Nutrition. 129: 1454S–1456S. Fructooligosaccharides and Lactobacillus acidophilus Modify Gut Microbial Populations, Total Tract Nutrient Digestibilities and Fecal Protein Catabolite Concentrations in Healthy Adult Dogs. J. Nutrition. 132: 3721–3731.
Karlin D.A., A.J. Mastromarino, R.D. Jones, J.R. Stroehlein and O. Lorentz. 1984. Fecal skatole and indole and breath methane and hydrogen in patients with large bowel polyps or cancer. J Cancer Res. Clinic Oncology. 109:135-141.
Lin H.C. and W.J. Visek. 1991. Large intestinal pH and ammonia in rats: dietary fat and protein interactions. J. Nutrition. 121: 832–843.
Schiffman S.S. 1998. Livestock Odors: Implications for Human Health and Well-Being. J. Animal. Sci. 76:1343–1355.
Silverman S.J. and A.W. Andrews. 1977. Bile acids, comutagenic activity in the Salmonella-mammalian microsome mutagenicity test. J. Nat. Cancer Inst. 59:1557–1559.
Stephen T.C., M.R. Roberto and J.W. Martin. 2008. Bacillus subtilis Spores Germinate in the Chicken Gastrointestinal Tract. Applied and Environmental Microbiology. 74(16): 5254-5258.
Swanson K.S., C.M. Grieshop, E.A. Flickinger, L.L. Bauer, J. Chow, B.W. Wolf, K.A. Garleb and G.C. Fahey, Jr. 2002.
Tam N.K., N.Q. Uyen, H.A. Hong, Le H. Duc, T.T. Hoa, C.R. Serra, A.O. Henriques, S.M. Cutting. 2006. The Intestinal Life Cycle of Bacillus subtilis and Close Relatives. J. Bacteriology. 188(7): 2692–2700.
Yumoto I., K. Hirota , Y. Nodasaka, Y. Tokiwa and K. Nakajima. 2004. Bacillus asahii sp. nov., a novel bacterium isolated from soil with the ability to deodorize the bad smell generated from short-chain fatty acids. International journal of systematic and evolutionary microbiology. 54(6):1997-2001.


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